2015    №1  №2  №3  №4

№1

Е.I. Shniukova, E.K. Zolotareva

DIATOM EXOPOLYSACCHARIDES. A REVIEW

Diatom exopolysaccharides (EPS) are bioactive components released into the environment. As an important component of marine phytoplankton, diatoms produce up to a quarter of the annual primary organic matter. The results of EPS studies highlighting the important role of diatom EPS in the global carbon cycle are analyzed. The main types of extracellular carbohydrates, monosaccharide composition of EPS, and dependence of their production on algal growth rate, light conditions and nutrient contеnt in the environment are discussed.

G.S. Minyuk, E.S. Chelebieva, I.N. Chubchikova

SECONDARY CAROTENOGENESIS OF THE GREEN MICROALGA BRACTEACOCCUS MINOR (CHLOROPHYTA) IN A TWO-STAGE CULTURE

Morphometric and physiologic-biochemical characteristics as well as the productivity of the green terrestrial microalga Вracteacoccus minor (Chodat) Petrová were investigated in a two-stage batch culture. The specific adaptive responses, which the microalga developed under the experimentally induced secondary carotenogenesis were: high cellular resistance to sodium acetate, the accumulation of a multicomponent mixture of secondary ketocarotenoids with the dominance of astaxanthindiesters (37–42% of the total carotenoids) and a large lipid content in algal biomass (53–63% of dry matter).

Yu.P. Kopytov, A.S. Lelekov, R.G. Gevorgiz, M.V. Nekhoroshev, T.M. Novikova

METHOD OF COMPLEX ANALYSIS OF BIOCHEMICAL COMPOSITION OF MICROALGAE

The method of complex analysis of biochemical composition of microalgae culture is proposed. The method allows to define dry weight on oxidability of biomass, content of chlorophylls, carotinoids, total proteins, carbohydrates, lipids. Use of the given method allows essentially to save time and reactants at the analysis since it is carried out by a micromethod.

A.A. Begun, L.I. Ryabushko, A.Yu. Zvyaginsev

BACILLARIOPHYTA OF PERIPHYTON OF NAVIGATION BUOYS IN THE POSYET BAY AREA (THE SEA OF JAPAN, RUSSIA)

Species composition of diatoms overgrowing navigation buoys in water areas of Posiet Bay of the Sea of Japan adjacent to the Far East Marine State Nature Biosphere Reserve is studied. Totally 93 species and intraspecies taxa of diatoms from Bacillariophyceae (61), Coscinodiscophyceae (22) and Fragilariophyceae (10) are registered. 43 taxa are first cited for the reserve. Most of the coves of the Bay are characterized by high species diversity (73 species) of periphyton Bacillariophyta and the dominance of β-mezosaprobionts, indicators of moderate organic pollution. In the cove of Trinity low species diversity (24) and the predominance of α-mezosaprobionts were recorded, largely due to the influence of anthropogenic pollution caused by port of Zarubino and fish factory.

N.Yu. Shoman, A.I. Akimov

THE COMBINED INFLUENCE OF LIGHT INTENSITY AND TEMPERATURE ON ORGANIC CARBON TO CHLOROPHYLL A RATIO IN THREE SPECIES OF MARINE BACILLARIOPHYTA

The combined effect of light and temperature on the change in the intracellular ratio of organic carbon to the chlorophyll a (C/chl. a) was studied in three species of diatoms: Phaeodactylum tricornutumBohlin, Nitzschia sp. 3 and Skeletonema costatum Cleve. At the optimum temperature of 20° C value of the C/chl. a ratio varies from 15 to 60–70 all over the studied light range in Ph. tricornutum and Nitzschia sp. 3, and up to 350 μE·m^-2·s^-1 in S. costatum. The dependence of this ratio on the light is described by hyperbolic curve. Temperature decrease results in increase both of the C/chl. a ratio in algal cells and increasing the initial tangent of the slope of the C/chl. a ratio curve. Inhibiting light combined with low temperature cause a decrease in the functional activity of algae, a decrease in the rate of synthesis of chl. a against the background of its enhanced photo-oxidation and, as a consequence, a sharp increase of the C/chl. a ratio. All studied diatom species are characterized by similar nature of light and temperature dependence of C/chl. a; however, in S. costatum the curve of C/chl. a ratio formed the plateau at lower light intensities while photo-oxidation of chlorophyll took place with an increase in luminance.

Е.V. Borisova, A.B. Gromakova

NEW LOCATION OF CHARA TOMENTOSA L. (CHARALES, CHAROHYCEAE) IN UKRAINE

The data on new location of the rare species for Ukrainian algal flora Chara tomentosa are reported. The morphological description, characteristics of ecology and preliminary assessment of its sozological status are given.

S.I. Genkal, E.V. Lepskaya

MATERIALS TO THE FLORA OF BACILLARIOPHYTA IN LAKE KRONOTSKOYE (THE KAMCHATKA PENINSULA, RUSSIA)

First electron microscopy study of Bacillariophyta,a major component of Lake Kronotskoye phytoplankton, revealed more than 80 taxa of diatoms. Among them two species are new for the flora of Russia (Cavinula thoroddsenii (Foged) Lange-Bert. and Navicula jentzschii Grunow), 10 forms were identified only to the genus. Genera Cymbella Agardh., Pseudostaurosira Williams & Round, Gomphonema Ehrenb., Planothidium Round & Bukht. and Navicula Bory are richest in species. Dominating species of phytoplankton are represented by pennate (Asterionella Hassall) and centric (Aulacoseira Thw., Handmannia M. Perag.) diatoms.

S.V. Skrebovska, I.Yu. Kostikov, P.M. Tsarenko

SCENEDESMUS BASILIENSIS R. CHODAT IN THE SCENEDESMACEAE (CHLOROPHYTA) SYSTEM

Comparative analysis of 18S rDNA partial sequence of authentic strain of Scenedesmus basiliensis subculture (ACKU 646-06) showed its identity with similar sequences of a number of strains of Acutodesmus obliquus (Turpin) P. Tsarenko. This result confirms status of S. basiliensis as a synonym of A. obliquus.

Т.N. Yatsenko-Stepanova, М.Е. Ignatenko, E.A. Selivanova, N.V. Nemtseva

ADDITION TO THE ALGAL FLORA FOR ORENBURG REGION

The results of algal flora study of saline and fresh water bodies of the Orenburg region were presented. 70 species and infraspecific taxa new for algal flora of the Orenburg region were found and belonged to 5 divisions. The data on ecological and geographical characteristics of the identified taxa are given.

K.S. Solomonova, V.S. Mukhanov

CYTOMETRIC METHOD FOR DETERMINING THE POTENTIAL GROWTH RATE OF PHYTOPLANKTON ON THE MITOTIC INDEX

The effect of light intensity and light-dark cycle (12 : 12) on the percentage of cells with diploid DNA in monospecific cultures of microalgae Phaeodactylum tricornutum Bohlin, Nitzschia sp. 3, Prorocentrum pusillum Shiller, P. cordatum (Ostf.) Dodge, Isochrysis galbana Parke was studied by flow cytometry. It was shown that the proportion of cells in G2 phase was significantly higher in high-intensity lighting. In all species strong correlation (r² > 0.98) was revealed between the potential growth rate of algae determining by the mitotic index, and their specific production as measured by the increase in number. The possibility of flow cytometry use for cell cycle analysis and evaluation of potential growth rates of picophytoplankton and nanophytoplankton of the Black Sea. At three stations in Sevastopol and Quarantine bays potential growth rate of pico- and nanophytoplankton in the surface layer was 0.2–1.0 d^-1 (year-round sumpling in 2010 at 3 stations, totally 120 samples). Maximum values of this parameter corresponded to the number of annual peaks of phytoplankton number.

№2 (2015)

F.P. Tkachenko, I.I. Maslov

FATTY ACIDS OF TOTAL LIPIDS OF SPECIES GENUS OF CYSTOSEIRA C. AGARDH (BLACK SEA, СRIMEA)

The fatty acid composition of total lipids estuary and marine populations of Cystoseira barbata (Gooden. et Woodw.) C. Agardh and marine C. crinita (Desf.) Bory was investigated. It is shown that their composition and value are species-specific in nature. Wider adaptation to environmental conditions in C. barbata, compared to C. crinita, achieved by increased synthesis of unsaturated fatty acids and acid forming part sulpholipids.

B.A. Panah, F. Najafi, N. Soltani, R.A.Kh. Nejad, S. Babaei

BIODEGRADATION ABILITY AND PHYSIOLOGICAL RESPONSES OF CYANOBACTERIUM LEPTOLYNGBYA SP. ISC 25 UNDER NAPHTHALENE TREATMENT

Cyanobacteria (Cyanoprokaryota) have gained a lot of attention in recent years because of their potential applications in biotechnology. In this study the cyanobacterium Leptolyngbya sp. ISC 25 was identified as tolerating and effectively degrading naphthalene as a toxic compound in the environment. The cyanobacterium was treated with different concentra-tions of naphthalene. Physiological responses such as survival, Chlorophyll a content, photosynthesis rate and ammonium excretion were investigated in logarithmic phase of growth curve. The biodegradation ability of the cyanobacterium was measured by GC and GC/MS analysis. Results indicated that chlorophyll a concentration decreased with naphthalene increasing and was approximately zero in the presence of 1 % naphthalene, Phycobiliproteins content enhanced up to 0.2 % of naphthalene, but at higher concentra-tions decreased significantly. Photosynthesis rate and ammonium excretion decreased in all treatments. Results of GC analysis confirmed the degradation of naphthalene by Leptolyngbya sp. ISC 25 in comparison with control (without the cyanobacterium). The results of GC/MS analysis identified the products of naphthalene degradation by Leptolyngbya sp. ISC 25. Totally lower concentrations of naphthalene is not lethal for cyanobacterium Leptolyngbya sp. and this strain can biodegrade naphthalene to 2(4H)-benzofuranone-tetrahydro-trimethyl mainly.

F.B. Shkundina, D.I. Sahabutdinova

DYNAMIC OF AUTOTROPHIC PLANKTON EXAMPLE SITE RIVER BELAYА (REPUBLIC OF BASHKORTOSTAN, RUSSIA)

During period from 1985-1986 and 2010-2012 were compared flora planktonic algae and Cyanoprokaryota. The changes of dominant species and quantitative development of autotrophic plankton were investigated. Water quality were estimated using indices saprobity. Water quality deterioration noted in April.

A.A. Snigirоva, G.V. Kovaleva

DIATOM ALGAE OF SAND SPITS OF THE NORTH WESTERN PART OF THE BLACK SEA (UKRAINE)

The species diversity of diatom algae of mezophytopsammon in three areas of the north-western part of the Black Sea (Kinburn, Karolino-Bugaz, Budakskaya sand-spits) has been studied. 51 taxa (ranged below genus) of Bacillariophyta from 26 genera were identified. First for north-western part of the Black Sea 5 species were revealed and for the Budakskyi liman – 1 species. Most of found species (17) are motile (epipelic), and only 4 species are attached to the sand grains (epipsammic). With respect to the salinity the diatoms were equally represented by polyhalobes (35 %), mezohalobes (35 %) and oligohalobes (31 %). The greatest number of species was represented by alkaliphilic (88 %). High abundance of diatom algae from mezophytopsammon (up to 12 million cells per cm²) indicates its high role in productive processes of coastal ecosystems of the Black Sea.

A.B. Komissarov, L.G. Korneva

CHARACTERISTICS OF TVERTSA RIVER PHYTOPLANKTON (IVAN’KOVO RESERVOIR, RUSSIA)

The results of the studies on the algal flora of the Tvertsa River, the largest tributary to the Ivan’kovo Reservoir, conducted in 2009-2011 are presented. The chemical characterization of the river is given. Species composition and taxonomic structure of the flora are specified along with the ecological-geographical parameters of the planktic algae. Totally 381 taxa of the algae of the rank below genus are found in the river. It has been revealed that the basis of floristic diversity is composed of Chlorophyta, Bacillariophyta, Euglenophyta and Cyanoprokaryota. The similarity of various parts of the river is assessed based on the species composition of algae and relation of species richness of various groups of algae with abiotic factors. The continuity of the changes in the plankton flora along the distance from the river head to its mouth determined by the water chemical factors is revealed.

E.Yu. Mitrofanova, S.I. Genkal

SPECIES COPMPOSITION OF BACILLARIOPHYTA ON CLASPING-LEAVED PONDWEED IN LAKE TELETSKOYE (ALTAI MOUNTAINS, RUSSIA)

The results of electron-microscopic study of diatom algae composition on the submerged macrophyte Potamogeton perfoliatus L. clasping-leaved pondweed in two large bays of Lake Teletskoye are presented. A total of 40 species were revealed, of which only eight were found in both bays. It was defined that the composition of diatoms in Kamga Bay, which is larger in area and length, is more diverse than in Kyga Bay. It was assumed, that the lake pelagic zone can influence on this process due to smaller size of the latter.

L.N. Bukhtiyarova

CLASSIFICATION OF UNISERIAL STRIAE IN BACILLARIOPHY TA WITH BIPOLAR FRUSTULE

The striae are the main structural-functional units in the diatom frustule, however, their morphology is still insufficiently studied and poorly used in Bacillariophyta taxonomy. New criteria for the stria architecture analysis rely on the data from electron microscopy investigations. For the first time hierarchical classification of the striae diversity in the diatom bipolar frustules was proposed. It reflects species ontogeny and the influence of ecology in evolution of different striae. Striae types were determined according to areolae types and originally defined stria parameters. Morphology of areolae determines macroareolar, postmacroareolar, combined and polyareolar striae types which are considered as the consistent stages in evolution of the striae in bipolar frustule. Our concept on the derivation of prevail in Bacillariophyta polyareolar stria from the macroareolar stria through the intermediate stria types is supported by similar morphological stages and their consistency recorded in frustule ontogeny of the species from different systematic groups of Bacillariophyta with bipolar symmetry. Basal and interbasal stria types were defined in accordance with the stria location within a basal siliceous layer. Conical type of areola and polyareolar stria with conical areolae are described. Their distinction from poroid areolae and polyareolar stria with poroid areolae is discussed. Proportional, compact and distant kinds of striae are defined on the ratio between stria and interstria areas and their meaning in species biology and ecology is indicated. All the definitions of the frustule structural units are based on the concept of functional morphology of the diatom frustule suggested by the author.

A.F. Krakhmalnyi

GYMNODINIUM FEOFANIUM KRACHM. SP. NOV. (DINOPHYTA, DINOFLAGELLATA)

Diagnosis and description of Gymnodinium feofanium Krachm. sp. nov. from the pond in Feofania Park (Kiev, Ukraine) are given, this species  was found in April  2014. Cells 18.0-29.0 µm long and 15.72-34.08 µm wide, globose, slightly compressed dorso-ventrally. Epicone hemispherical, slightly asymmetrical. Hypocone reverse trapezoidal, cup-shaped. In the area of sulcus there is a protrusion of epicone, its length comprises two widths of the cingulum. Cingulum equatorial, descending, displaced 0.5-1.0 cingulum widths. Sulcus wide, V-shaped, located only on hypocone, reaches antapex. Chloroplasts numerous, fulvous, located at the periphery of the cell. Nucleus large, usually located in the center. Stigma red colored. Small vacuoles are present in the cytoplasm.

№3 (2015)

S.I. Genkal, T.A. Chekryzheva

NEW DATA TO MORPHOLOGY, TAXONOMY AND DISTRIBUTION EUNOTIA BICONSTRICTA (GRUNOW) LANGE-BERT. (BACILLARIOPHYTA)

The study of extensive materials from the rivers and lakes of Karelia and Western Siberia using scanning electron microscopy revealed significant morphological variability of quantitative (valve length and width, the number of striae and areolae in 10 μm) and qualitative (shape of valve and terminal raphe fissures) features. Based on the original and published data, it allowed extending the description of Eunotia biconstricta and referring some taxa as synonyms.

F.P. Tkachenko, I.P. Tretyak

MACROPHYTOBENTHOS OF BOTANICAL RESERVATION OF NATIONAL SIGNIFICANCE «ZERNOV’S PHYLLOPHORA FIELD» (UKRAINE)

Species composition of macrophytobenthos botanical reservation «Zernov’s Phyllophora Field» was presented. There are 24 species, among them Phaeophyta (6), Rhodophyta (12) and Chlorophyta (6). We observe a noticeable trend towards recovery the thickets of phyllophora and the species composition of phytobenthos field on the former territory of FPZ.

M.N. Khalid, Z. Riaz, M. Shameel

STUDIES ON BIOACTIVITY OF CHARA BRAUNII C.C. GMELIN (CHAROPHYTA)

Chara braunii a freshwater green algae belonging to the division Charophyta was collected from different freshwater habitats of Faisalabad region of Pakistan. Its antifungal activity was determined by using agar well diffusion method against ten fungal cultures. The results showed a significant response and promising antifungal activity of Ch. braunii against the tested fungal species. The present research work is highly significant in terms of understanding the response of important new natural sources of bioactive compounds for pharmaceutical industry and it is first comprehensive report from Faisalabad region.

A.A. Begun, Yu.N. Elkin

THE ROLE OF BACILLARIOPHYTA IN FEEDING OF CLYPEASTEROIDS SCAPHECHINUS MIRABILIS (AGAZZIS, 1863) AND ECHINARACHMUS PARMA (LAMARCK, 1818) (ECHINOIDEA, CLYPEASTEROIDA)

Algal flora of the coarse-grained sandy substrate and faeces of clypeasteroids Scaphechinus mirabilis (Agazzis, 1863) and Echinarachmus parma (Lamarck, 1818) living in Troitsa Bay, Sea of Japan, were studied. A total of 50 species of microalgae were recorded, most of them (42) belong to Bacillariophyta. In the clypeasteroids faeces, diatoms were less diverse (27 species) when compared with a sandy substrate (50); most of the cells had chloroplasts, which provided evidence for selection of the clypeasteroids during feeding. High similarity of algae flora in S. mirabilis and E. parma faeces (0.97) indicated the similarity in food specialization of these clypeasteroids sharing the same ecological niche. The data largely confirmed the assumption of the essential role of diatoms in the feeding of clypeasteroid S. mirabilis.

A.G. Okhapkin, E.L. Vodeneeva, O.O. Bondarev

THE COMPOSITION OF CYANOPROKARYOTA IN THE PLANKTON OF CHEBOKSARY RESERVOIR (NIZHNIY NOVGOROD, RUSSIA)

According to the recent data on the taxonomy and nomenclature of taxa a revision of blue-green algae composition (Cyanoprokaryota, Cyanophyta) of Cheboksary reservoir was conducted. It was found 73 species from 27 genera, 9 families and 3 orders. The main part of the Cyanoprokaryota flora was formed by representatives of genera Dolichospermum, Merismopedia, Chroococcus, Microcystis, Rhabdogloea, Oscillatoria. Taxonomic, ecological and geographical characteristics of blue-green algae were given. Main changes in the taxonomic structure of this group of algae after the Volga River flow regulation were marked. The most important of them were the increasing of total species richness of Cyanophyta, the appearance of new species of five genera and the increasing diversity of the genus Dolichospermum nearly twice.

L.M. Terenko†, D.A. Nesterova

CYANOPROKARYOTA OF THE PLANKTON OF THE NORTHWESTERN BLACK SEA (UKRAINE)

The results of the study of species composition, abundance and biomass of planktic Cyanoprokaryota of the northwestern part of the Black Sea are presented. Totally 63 species of cyanophytes were recorded. Aphanocapsa salina, Aph. salina, Dolichospermum affine, Anabaenopsis elenkinii, A. seriata, Pseudanabaena limnetica, Oscillatoria limosa, Microcystis wesenbergii and Planktothrix sp. are first cited for the Black Sea. In recent years mass development of Cyanoprokaryota causes water blooms in the summer and early autumn worsening water quality. Dominant species are Anabaena spiroides, Aphanizomenon flosaquae, Nodularia spumigena, Oscillatoria kisselevii, Spirulina laxissima and Microcystis aeruginosa. The dynamics of abundance and biomass, a share of Cyanoprokaryota in the structure of phytoplankton and possible causes of water bloom are discussed.

O.S. Ogienko

TAXONOMIC COMPOSITION OF DIATOM ASSEMBLAGES (BACILLARIOPHYTA) FROM THE QUATERNARY DEPOSITS, THE SCOTIA SEA (ANTARCTIC)

The taxonomic composition of the diatom assemblages in the Upper Quaternary sediments of the Southern Scotia Sea is studied. The existence of species in the sea-floor deposits typical for modern plankton associations of this region is demonstrated, which allows to characterize the paleoenvironmental conditions of the basin at the time of the complex formation as similar to the modern environment. The lower stratum of the sediments, among the Late Quaternary species, is characterized by the presence of valves of more ancient Middle Miocene species, which indicates at the re-deposition processes, taking place in Late Quaternary. The research results of the taxonomic composition and ecological structure of the re-deposited diatomaceous complexes are presented and the analysis of the paleoenvironment of the Miocene basin is performed.

О.N. Krivosheya, A.A. Krivenda

NEW AND RARE SPECIES OF BACILLARIOPHYTA FROM THE BASINS OF REGIONAL LANDSCAPE PARK «NYZHNYOVORSKLYANSKY» (UKRAINE)

Morphological, chorological and ecological data are given for the new and regionally rare diatoms of Ukrainian algal flora. Short characteristics and microphotographs are given for the each of new and interesting diatom species, which were found in periphytic habitats of the basins of regional landscape park “Nyzhnyovorsklyansky”. Thirty species were firstly recorded for the Ukrainian Forest-Steppe zone, 6 – for the Left-bank Forest-Steppe region. Extended morphological characteristics are given for the 6 species which were new for the algal flora of Ukraine: Caloneis macedonica Hust., Encyonema lange-bertalotii Krammer, E. vulgare Krammer, Navicula heimansioides Lange-Bert., N. streckerae Lange-Bert. & Witkowski in Witkowski et al., Placoneis minor (Grunow) Lange-Bertalot in Metzeltin et al.

G.G. Minicheva

NEW INVIDER IN THE BLACK SEA: BROWN ALGAE CHORDA TOMENTOSA LYNGB.

In April 2015, in the northwestern part of the Black Sea (Ukraine, Odessa Bay, Cape Bolshoy Fountain) the brown algae Chorda tomentosa Lyngb. 1819, a representative of the order Laminariales Mig., 1909 was founded. Fixed that species is a new invider in Black Sea and the sole representative of the order of Laminariales in this area.

E.A. Romanenko, I.V. Kosakovskaya, P.A. Romanenko

PHYTOHORMONES OF MICROALGAE: BIOLOGICAL ROLE AND INVOLVEMENT IN THE REGULATION OF PHYSIOLOGICAL PROCESSES. PT I. AUXINS, ABSCISIC ACID, ETHYLENE

The literature data about the features of biosynthesis, chemical structure, interaction and impact on the growth and development of microalgae of different classes of phytohormones have been analyzed and summarized. The information about the divisions and species of algae with measured auxins, abscisic acid (ABA) and ethylene is provided. Specific features of regulation of microalgae biomass production, cell division processes, resistance to abiotic stressors by exogenous auxins, ABA and ethylene are discussed. The main directions and tasks for further microalgae phytohormones researches, perspectives of their using in the development of biotechnological approaches in the agricultural industry, medical and pharmaceutical research are analyzed.

№4 (2015)

G.M. Palamar-Mordvintseva, P.M. Tsarenko

ALGOFLORISTIC ZONING OF UKRAINE

General approaches and criteria of the floristical zoning are analyzed. Basic principles and criteria for the algofloristic zoning of Ukraine are proposed. A variant of the delimitation of this territory is given. Main characteristics of the taxonomical and floristical diversity of the desmidian and green coccoid algae from Ukrainian continental freshwaters are discussed. Basic patterns of the regional distribution of these groups of algae are also considered. Algofloristic status of the territory of Ukraine is given according to the results of algofloristic studies of Ukraine (on the example of aforementioned groups of algae) and comparative studies of the floras of several European countries. We consider it a s a part of algofloristic Eastern European province and divide onto 5 algofloristic subprovinces, 16 regions and 26 floristic districts. Characterization of different algofloristic chorions – up to the locality – is given (on the example of Pripyat-Desna algofloristic subprovince).

S.I. Genkal, O.P. Bilous

CENTRIC DIATOMS (CENTROPHYCEAE) OF THE SOUTHERN BUG RIVER LOWER PART (UKRAINE)

The study of the Southern Bug River lower part of the phytobenthos by scanning electron microscopy revealed 16 representatives of diatoms Centrophyceae class of 8 genera, wherein the species composition of centric diatoms significantly expanded by taxa of species (10) and genera (Conticribra, Thalassiosira) rank.

I.S. Suprun

PALEOECOLOGICAL RECONSTRUCTIONS OF PALEOCENE SEDIMENTS FROM THE TETHYAN PROVINCE BASED ON CALCAREOUS NANNOFOSSILS

The paper includes the results of paleoenvironmental reconstructions of Paleocene sections of some areas of Tajikistan, Egypt, Ukrainian Carpathians and the NW shelf of the Black Sea based on calcareous nannofossils. The obtained results enabled to reconstruct the paleobionomic conditions of the Paleocene Basins. Almost the entire research area during the Paleocene was characterized by warm water (tropical and subtropical) conditions.

K. Aisha, M. Shameel

TAXONOMY OF THE GENUS IYENGARIA BØRGESEN (PHAEOPHYCOTA) FROM COASTAL WATERS OF KARACHI, PAKISTAN

Globular, hollow and stellate thalli of the brown algal genus Iyengaria were collected from various coastal areas of Karachi and taxonomically investigated. This study revealed the presence of two species i.e. I. stellata (Børgesen) Børgesen and a new species, I. lobocylindrica Aisha et Shameel. The new species is characterized by the thin walled thallus, hollow projections, cortex not differentiated into inner and outer portion, absence of phaeophycotaen hairs, and thin walled plurilocular sporangia with rectangular locules. Its thalli are lobed in early stages but gradually become cylindrical.

E.S. Gusev

A NEW SPECIES OF THE GENUS MALLOMONAS (SYNURALES, CHRYSOPHYCEAE) FROM VIETNAM

Mallomonas paragrandis E.S. Gusev sp. nov. is described from Zua River, located in Khanh Hoa Province, Central Viet Nam. The description is based on silica-scale morphology studied by means of transmission and scanning electron microscopy. New species has oval or obovoid scales with evenly distributed large pores in proximal half of the scales. Distal part is covered with hexagonal reticulation with minute pores inside each mesh and papillae in the angles of the meshes. New species belongs to the Section Planae and closely related to Mallomonas sorohexareticulata Jo, Shin, Kim, Siver & Andersen, M. hexareticulata Jo, Shin, Kim, Siver & Andersen and M. matvienkoae Asmund & Kristiansen var. grandis Dürrschmidt & G. Cronberg. Results of morphological comparison were approved by molecular analysis on the basis of nuclear 18S and 28S rDNA and plastid rbcL genes.

E.V. Borisova & D.N. Iakushenko

THE FIRST FINDING OF CHARA RUDIS (A. BRAUN) LEONH. (CHARALES, CHAROPHYTA) IN UKRAINE

A new locality of Chara rudis has been found in the Ivano – Frankivsk Region (Ukrainian Carpathians) in summer 2008. It forms charophyte single-species meadows in a freshwater pond at a depth of 0.3–0.5 up to 1 m on silt but on solid substrate with a surface cover of 75–95%. It is the first finding of this alga in Ukraine. The specimens are described; distribution and ecology of C. rudis as well as its taxonomical status are discussed.