IBK Mushroom Culture Collection


The curator of the IBK – Bisko Nina, Dr. Sci., Main scientist, professor, Laureate of the State Prize of Ukraine

Phone: +38 (044) 234-12-32

E-mail: bisko_nina@ukr.net



Over 10 thousand species of Basidiomycota and Ascomycota are macrofungi, or mushrooms. Mushrooms play an important role in natural environment decomposing a huge lignocellulose biomass and plant litter, forming mycorrhiza, etc. They also have considerable economic significance as objects of mushroom growing industry and as a source of pharmacological substances with oncostatic, immunomodulating, radioprotective, antiviral and other properties, dietary supplements, enzymes, antibiotics, etc.

Regarding conservation of mycobiota outside natural habitats of mushrooms, ex situ culture collections are crucial in maintaining the gene pool of macrofungi in pure culture. The Mushroom Culture Collection (acronym IBK) was established at the M.G. Kholodny Institute of Botany, NASU in 1966 on the basis of the Department of Mycology. A founder and first curator of the IBK Collection was Dr. Professor A.S. Bukhalo (1932-2014) who defined the strategy for development of collection funds and proposed basic principles of selection and preservation of mushroom diversity.

The Collection includes a total number of 1340 strains belonging to 265 species of Basidiomycota and Ascomycota. The IBK Collection is the largest official specialized culture collection of macromycetes in Ukraine and one of the largest in number of species and strains in Europe. According to Decree of the Cabinet of Ministers of Ukraine № 1709 of 19 December 2001, the Mushroom Culture Collection of the M.G. Kholodny Institute of Botany was registered as a scientific object of national heritage of Ukraine.

The Culture Collection activities are focused on preservation of genofund of macrofungi in pure culture and fundamental research on biology and biotechnology of edible and medicinal mushrooms. Dikaryotic strains of macromycetes from different taxonomic and ecological groups of broad geographical origin are maintained in the Collection. The collection is of great environmental importance for ex situ conservation of the fungal genetic resource. Introduction to culture and preservation of rare and endangered species of fungi in Ukraine are emphasized, particularly those species which are listed in the Red Data Book of Ukraine. Among them, there are ascomycetes Morchella steppicola Zerova, M. crassipes (Vent.) Pers., Gyromitra slonevskii Heluta and basidiomycetes Fomitopsis officinalis (Vill.) Bondartsev et Singer, Hericium coralloides (Fr.) Gray, Grifola frondosa (Dicks.) Gray, Sparassis crispa (Wulfen) Fr. maintained in the IBK Collection. In addition, strains of Pleurotus nebrodensis (Inzenga) Quel., until recently the only representative of the Kingdom Fungi in the IUCN Red List of Threatened Species, are preserved in the Collection. Due to sufficient strain diversity, it is possible to reintroduce rare species into natural habitats where they have been extinct for various reasons.

A special attention has been paid to the introduction of species and strain diversity of edible and medicinal mushrooms. Unlike other macromycetes collections, in the IBK Collection contains a large number of strains commonly cultivated worldwide by producers of edible mushrooms and dietary and pharmacological substances. They belong to the genera Pleurotus (Fr.) P. Kumm., Agaricus L., Lentinus Fr., Oudemansiella Speg., Flammulina P. Karst., Hericium Pers., Piptoporus Karst., Omphalotus Fayod, Schizophyllum Fr., Ganoderma P.Karst., Trametes Fr., Laetiporus Murrill, Lycoperdon Pers., Coprinus Pers., Macrolepiota Singer, etc. and are represented in the Collection by a wide diversity of strains.

Culture collection of mushrooms is an important resource for development of mushroom growing in Ukraine and biotechnologies of dietary treatment and prevention supply, food supplements, pharmaceutical and biologically active substances. Cultures of 123 species with known pharmacological properties used in international folk and traditional medicine are represented in the Collection.

Methods of storage of pure cultures of ascomycetes and basidiomycetes are determined by peculiarities of morphology, ecological and biological properties of the fungi that develop in culture mainly as asporous vegetative mycelium. Isolation of pure cultures from fruit body tissue or basidio- and ascospores are made using conventional and modified methods. For isolation and preservation of cultures, wort, malz, compost, potato-dextrose and other agar media, including added herbal extracts, are used. Cultures are preserved in refrigerator at 4±1 °C.

For species identification of basidiomycetes in vegetative stage of their development, specific criteria for identification and verification of macromycetes of certain taxonomic and ecological groups in culture are required. Peculiarities of mycelium growth and formation of fruiting bodies in culture are investigated within special program developed by the Collection staff. This program includes research on cultural-morphological characteristics using scanning electron microscopy, cultivation conditions, physiological and biochemical characteristics of strains that can be used to determine species identity of the culture.
It has been established that during cultivation period on reference agar nutrient medium, taxonomically significant at species level are: ability to form teleomorph stage; presence and type of asexual sporification; presence, shape and location on the mycelium of clamp connections, chlamydospores; formation of crystals inlays and abnormal structures on hyphae; type of filamentous colony and its radial growth rate; nature of the colored test reactions in presence of certain enzymes.
The Collection staff has developed methods of passaging, choosing selective nutrient media for some species with higher requirements for nutrients and microscopic visual system control cultures in absence of extraneous microflora which allow to maintain the viability of cultures in the Collection, including enzymatic and physiological activity, for decades.
For the development of new methods of cultivation and original culture media, based on selected strains from the IBK Culture Collection, the staff of the Department of Mycology of the M.G. Kholodny Institute of Botany was awarded twice by the State Prize of Ukraine in Science and Technology (1989, 2005), Award of the National Academy of Sciences of Ukraine, Academy of Sciences of Belarus and the Academy of Sciences of Moldova (2009). The staff of the Collection has organized the First International Conference  “Perspectives of Medicinal Mushrooms in Care Health and Nutrition in 21st century” devoted to issues of biology and biotechnology of medicinal mushrooms, held in Kiev in 2001.
The Collection regularly integrates new strains. Isolation of macromycetes into the culture collection to enrich the fund is performed in the field during expedition trips and in laboratory. All mycological material before the isolation is identified by mycologists, experts in taxonomy of relevant groups of fungi. The majority of cultures were isolated from the natural material in Ukraine, Russia, Belarus, Czech Republic, Israel and the USA. Some cultures were obtained from other collections, institutions and mycologists.
The Culture Collection of mushrooms provides consulting on methodological issues and identification of cultures, exchanges of culture collections around the world. Collection IBK is a major scientific and information basis for fundamental and applied research, training of master, bachelor and theses in biology and biotechnology of macromycetes in research institutions and higher educational institutions of Ukraine, Belarus, Russia, Moldova, Latvia and other countries. For the last 50 years, the species and strains of IBK Collection were the research objects for 25 PhD and 8 DrSci theses, as well as the basis for publication of 11 monographs, numerous articles in national and international journals.

The most important publications which include information about the Collection and investigated strains are cited in the IBK Catalogue (2016) and the list of publications below.

The mushroom cultures maintained by IBK has been included in the World Directory of Collections of Cultures of Microorganisms of the World Data Centre (WDCM), the curator of the collection is Dr. Bisko N.A., access number 1152, as well as in the Global Biodiversity Information Facility (GBIF).



  1. Buchalo A.S. Pure culture of Higher Basidiomycetes. In: Methods of experimental mycology. – K.: “Naukova dumka”, 1982. – P. 448-461. (Russ.)
  2. Buchаlo A.S. Higher edible Basidiomycetes in pure culture. – K.: “Naukova dumka”, 1988. – 144 p. (Russ.)
  3. Mitropolskaya N.Yu., Buchalo A.S. Higher Basidiomycetes culture collection at N.G. Kholodny Institute of Botany of the Academy of Sciences of Ukraine (Kiev) // Ukr. Botan. Journ. – 1994. – V. 51, № 1. – P. 125-130. (Ukr.)
  4. Buchalo A.S., Katchurovska V.P., Mitropolskaya N.Yu. Culture characteristic of rare species of macromycetes (Basidiomycotina) // Ukr. Botan. Journ. – 1995. – V. 52, № 1 – P. 104-113. (Ukr.)
  5. Weis A.L., Solomko E.F., Buchalo A.S., Wasser S.P., Mitropolskaya N.Yu., Grigansky A.Ph.,  Gorovits E.L. Cultural stady and illudin S production of medicinal mushroom Omphalotus olearius (DC.: Fr.) Fay. (Agaricales s.l.) from Israel // Int. J. of Med. Mush. – 1999. – V. 1, № 1. – P. 93-103.
  6. Solomko E.F., Lomberg M.L., Mitropolska N.Yu., Cholovska E.V. The growth of some medicinal macromycetes species on the different nutritious media // Ukr. Botan. Journ. – 2000. – V. 57, № 2. – P. 119-126. (Ukr.)
  7. Mitropolskaya N.Yu., Buchalo A.S. Culture Collection of Mushrooms at N.G. Kholodny Institute of Botany NASU (Ukraine) // Int. J. of Med. Mush. – 2001. – V. 3, № 2-3. – P. 181.
  8. Buchalo A.S. Studies on Medicinal Mushrooms at the National Ukrainian Culture Collection // Int. J. of Med. Mush. – 2001. – V. 3, № 2-3. – P. 93.
  9. Buchalo A.S., Mitropolskaya N.Yu. Investigations at the Ukrainian Culture Collection of Edible and Medicinal Mushrooms // Int. J. of Med. Mush. – 2002. – V. 4, № 3. – P. 245-254.
  10. Fomina V.I., Mitropol’skaya N.Yu., Bis’ko N.A., Shevtsova L.V. The screening of high productive strains of Lentinus edodes // Mycology and phytopathology. – 2003. – V. 37, № 2. – P. 60-65. (Russ.)
  11. Bisko N.A., Mitropolskaya N.Yu. Some Biologically Active Substances from Medicinal Muchroom Ganoderma lucidum (W. Curt.:Fr.) P. Karst. (Aphyllophoromycetideae) // Int. J. of Med. Mush. – 2003. – V. 5, № 3, – P. 301-305.
  12. Buchalo A.S., Diduch M.Ya. Micromorphological characteristics of culinary-medicinal Mushroom and Fungi cultures // Int. J. of Med. Mush. – 2005. – V. 7, № 1&2. – P. 249-262.
  13. Buchalo A.S., Didukh M.Ya., Mykhaylova O.B., Lynovitska V.M. Microstructures in Medicinal Mushroom Cultures // Int. J. of Med. Mush. – 2005. – V. 7, № 3. – P. 389-390.
  14. Mykchaylova O.B., Buchalo A.S. Morphological characteristic of morels pure cultures Morchellaceae (Ascomycetes) on agar nutrional media // Ukr. Botan. Journ. – 2005. – V. 62, № 4. – P. 500-508. (Ukr.)
  15. Mykchaylova O.B., Buchalo A.S. Mycelial microstructures in pure cultures of the representatives of Morchellaceae (Ascomycota) // Ukr. Botan. Journ. – 2005. – V. 62, № 6. – P. 790-796. (Ukr.)
  16. Poyedinok N.L., Mykсhaуlova O.B., Shcherba V.V., Buchalo A.S., & Negriyko A.M. Light regulation of growth and biosynthetic activity of Ling Zhi or Reishi medicinal mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae) in pure culture // Int. J. of Med. Mush. – 2008. – V. 10, № 4. – P. 369-378. DOI: 10.1615/IntJMedMushr.v10.i4.100
  17. Buchalo A., Mykchaylova O., Lomberg M., and Wasser S.P. Microstructures of vegetative mycelium of macromycetes in pure cultures // Eds. P.A. Volz and E. Nevo. – Kiev: Alterpress. – 2009. – 224 p.
  18. Smirnov D.A., Babitskaya V.G., Puchkova T.A., Shcherba V.V., Bisko N.A., Poyedinok N.L. Some Biologically Active Substances from a Mycelial Biomass of Medicinal Caterpillar Fungus Cordyceps sinensis (Berk.) Sacc. (Ascomycetes) // Int. J. of Med. Mush. – 2009. – V. 11, N 1. – P. 69-76.
  19. Anischenko I.M., Gurinovich N.V., Mitropolskaya N.Yu., Klechak I.R. Using Information Databases for the Study of Strains of Biotechnologically Valuable High Basidiomycetes // Naukovi visti. – 2010. – № 3. – С. 5-9.
  20. Antonenko L.O., Bisko N.A., Mytropolska N.Yu., Klechak I.R. Boundary Temperatures for Cultivation of Basidiomycetess Mushrooms of Genus Coriolus Quel. in the Surface Culture // Naukovi visti. – 2011. – № 3. – С. 7-11.
  21. Dyakov M.Y., Kamzolkina O.V., Shtaer O.V., Bisko N.A., Poyedinok N.L., Mykchaylova O.B., & Efremenkova O.V. Morphological characteristics of natural strains of certain species of basidiomycetes and biological analysis of antimicrobial activity under submerged cultural conditions // Microbiology. – 2011. – V. 80, № 2. – P. 274-285.
  22. Бухало А.С., Бабицкая В.Г., Бисько Н.А., Вассер С.П., Дудка И.А., Митропольская Н.Ю., Михайлова О.Б., Негрейко А.М., Поединок Н.Л., Соломко Э.Ф. Биологические свойства лекарственных макромицетов в культуре / Под ред. чл.-кор НАН Украины С.П. Вассера. – К.: «Альтерпрес», 2011. – Т. 1. – 212 с.
  23. Бисько Н.А., Бабицкая В.Г., Бухало А.С., Круподерова Т.А., Ломберг М.Л., Михайлова О.Б., Пучкова Т.А., Соломко Э.Ф., Щерба В.В. Биологические свойства лекарственных макромицетов в культуре / Под ред. чл.-кор НАН Украины С.П. Вассера. – К.: «Институт ботаники им. Н.Г. Холодного НАН Украины», 2012. – Т. 2. – 459 с.
  24. Macromycetes: medicinal properties and biological peculiarities / Ed. S.P. Wasser. – K., 2012. – 285 p.
  25. Moldavan M.G., Grodzinskaya A.A., Wasser S.P., Solomko E.F., Syrchin S.A., Gryganskyi A.P., Kolotushkina E.V., Lomberg M.L. Neurotropic action of macromycetes // “Macromycetes: medicinal properties and biological peculiarities” / Ed. S.P. Wasser. – K., 2012. – Р. 46-106. (In Russian)
  26. Poyedinok N., Mykchaylova O., Tugay T., Tugay A., Negriyko A., Dudka I. Effect of Light Wavelengths and Coherence on Growth, Enzymes Activity, and Melanin Accumulation of Liquid-Cultured Inonotus obliquus (Ach.:Pers.) Pilát. // Appl. Biochem. Biotechnol. – 2015. – 176. – P. 333-343.
  27. Poyedinok N., Negriyko A., Mykchaylova O. Effects of light wavelengths and coherence on basidiospores germination // Journal of Microbiology, Biotechnology and Food Sciences. – 2015. – V. 4, № 4. – P. 352-357.
  28. Lomberg M.L., Mykchaylova O.B., Bisko N.A. Mushroom culture collection (IBK) as a subject of national heritage of Ukraine // Ukr. Bot. J. – 2015. – V. 72, № 1. – P. 22-28.
  29. Vedenicheva N.P., Al-Maali G.A., Mytropolska N.Yu., Mykhaylova O.B., Bisko N.A., Kosakivska I.V. Endogenous cytokinins in medicinal Basidiomycetes mycelial biomass // Biotechnologia Acta. – 2016. – V. 9, № 1. – P. 55-63.
  30. Bisko N.A., Mustafin K.G., Suleimenova Zh.B., Akhmetsadykov N.N., Narmuratova Zh. Development for the physiologically active inoculum techniques and the study of the kinetic parameters of fungal biomass and biologically active substances synthesis // Europian Journal of Biomedical and Life Sciences. – 2016. № 3. – P. 4-10.
  31. Al-Maali G.A., Bisko N.A., Ostapchuk A.M. The effect of zinc citrate and zinc sulfate on the growth and biomass composition of medicinal mushroom Ganoderma lucidum // Mikologiya i Fitopatologiya. – 2016. – V. 50, № 5. – P. 313-317.
  32. С.П. Вассер, С.М. Бойко, Х. Вонг, K. Воронин, А.Ф. Григанский, А.А. Гродзинская, И.А. Дудка, Б. Кирхгоф, Е.В. Колотушкина, М.Л. Ломберг, М.Г. Молдаван, П. Педарзани, В. Сабаратнам, А.И. Самчук, Г.Г. Скибо, С.А. Сырчин, М. Фомина, П.Г. Ченг Макромицеты: лекарственные свойства и биологические особенности. Т. 2. / Под ред. проф. И. Габриэля. – К.: Наш формат, 2016. ‐ 261 с.
  33. Бісько Н.А., Ломберг М.Л., Митропольська Н.Ю., Михайлова О.Б. Колекція культур шапинкових грибів (IBK). – /Інститут ботаніки ім. М.Г. Холодного Національна Академія наук України. – Київ: «Aльтерпрес», 2016. − 120 с.: іл. 8.
  34. Myronycheva O., Bandura I., Bisko N., Gryganskyi A.P., Karlsson O. Assessment of the Growth and Fruiting of 19 Oyster Mushroom Strains for Indoor Cultivation on Lignocellulosic Wastes // BioResources, 2017. – V. 12, № 3. – P. 4606-4626. DOI: 10.15376/biores.12.3.4606-4626
  35. Mykchaylova O.B., Gryganskyi A.P., Lomberg M.L., Bisko N.A. The study of morphological and cultural properties of Sparassis crispa (Sparassidaceae, Polyporales) // Ukrainian Journal of Ecology. – 2017. – V. 7, № 4. – P. 550–558. DOI: 10.15421/2017_159
  36. Mykchaylova O.B., Bisko N.A., Sukhomlyn М.М., Lomberg М.L., Pasaylyuk М.V., Petrichuk Y.V., Gryganskyi A.P. Biological peculiarities of a rare medicinal mushroom Fomitopsis officinalis (Fomitopsidaceae, Polyporales) on agar media and plant substrates // Regul Mech Biosyst. – 2017. – V. 8, № 4. – P. 469-475. DOI: 10.15421/021772
  37. Vedenicheva N., Al-Maali G., Bisko N., Shcherbatiuk M., Lomberg M., Mytropolska N., Mykchaylova O.B., Kosakivska I.V. Comparative Analysis of Cytokinins in Mycelial Biomass of Medicinal Mushrooms // Int. J. of Med. Mush. – 2018. – V. 20, № 9.– P. 837-847. DOI: 10.1615/IntJMedMushrooms.2018027797
  38. Al-Maali G.A. The influence on different compounds of trace elements on the biomass and synthesis of exopolysaccharides of mycelium Trametes versicolor (Polyporaceae, Polyporales) // Biosystems Diversity. – 2018. – V. 25, № 4. – P. 289-296.
  39. Bisko N.A., Mytropolska N.Yu., Lomberg M.L., Mykchaylova O.B., Al-Maali G.A. The rare and biotechnologically important mushroom species in the IBK collection // In: Development of natural sciences in countries of the European Union taking into account the challenges of XXI century. Collective monograph. The book is compiled by: prof. dr hab. Witold Kłaczewski, doc. Henryk Stefanek, dr n. biol. Małgorzata Gorzel Lublin, Poland, 2018. – P. 21-37.
  40. Vedenicheva N.P., Al-Maali G.A., Mykchaylova O.B., Lomberg M.L., Bisko N.A., Shcherbatiuk M.M., Kosakivska I.V. Endogenous Cytokinins Dynamics in Mycelial Biomass of Basidiomycetes at Different Stages of Cultivation // Int. J. Biochem. Physiol. – 2018. – V. 3, № 2. – P. 1-7. DOI: 10.23880/IJBP-16000122
  41. Bisko N.A., Lomberg M.L., Mykchaylova O.B., Mytropolska N.Yu. Conservation of biotechnological important species diversity and genetic resource of rare and endangered fungi of Ukraine // Plant & Fungal Research, Institute of Botany, ANAS, Baku, Azerbaijan (December 2018). – 2018. – V. 1, № 1. – P. 18-27.
  42. Bisko N.A., Sukhomlyn M.M., Mykchaylova O.B., Lomberg M.L., Tsvyd N.V., Petrichuk Yu.V., Al-Maali G.A., Mytropolska N.Yu. Ex situ conservation of rare and endangered species in mushroom culture collections of Ukraine // Ukr. Bot. J. 2018. – V. 75, № 4. – Р. 338-347.
  43. Poyedinok N.L., Tugay T.I., Tugay A.V., Mykchaylova O.B., Sergiichuk N.N., & Negriyko A.M. Influence of nitrogen concentration on photoinduced growth, enzymatic activity and melanine synthesis by Inonotus obliquus (Ach.: Pers.) Pilát. // Biotechnologia Acta. – 2019. – V. 12, № 4. – P. 34-41. DOI: 10.15407/biotech12.04.034
  44. Mykchaylova O.B., Lomberg M.L., Bisko N.A. Verification and screening of biotecnologically valuable macromycetes species in vitro. In: Development of Modern Science: the Experience of European Countries and Prospects for Ukraine: monograph /edited by authors. 3rd ed. Riga, Latvia: Baltija Publishing. – 2019. – Р. 354-375.
  45. Al-Maali G.A., Vedenicheva N.P., Bisko N.A., Kosakivska I.V. Effect of microelements on cytokinins content in mycelial biomass of medicinal mushroom Trametes versicolor (Polyporaceae, Basidiomycota) // Ukr. Bot. J. – 2019. – V. 76, № 1. – P. 71-78.
  46. Medvediev D.G., Kerner A.O., Bondaruk S.V., Al-Maali G.A. Investigation of cultural features and fungicide resistance of the strains of Cladobotryum mycophilum (Hypocreales, Ascomycota), a causal agent of cobweb disease on button mushroom crops, newly recorded in Ukraine // Ukr. Bot. J. – 2019. – V. 76, № 2. – P. 121-131.
  47. Regeda L.V., Bisko N.A. Micromorphological characteristics of the species of Pholiota (Strophariaceae, Basidiomycota) in pure culture // Ukr. Bot. J. – 2019. –  V. 76, № 2. – P. 114-120.
  48. Grodzynska G., Lomberg M., Rahmetov D., Nebesnyi V. Biodegradation of energy plants substrates with culinary-medicinal mushrooms // Agroecological Journal. – 2019. – № 1. – Р. 85-89.
  49. Bisko N., Mustafin K., Al-Maali G., Suleimenova Zh., Lomberg M., Narmuratova Zh., Mykchaylova O., Mytropolska N., Zhakipbekova A. Effects of cultivation parameters on intracellular polysaccharide production in submerged culture of the edible medicinal mushroom Lentinula edodes // Czech Mycol. – 2020. – V. 72, № 1. – P. 1-17. DOI: 10.33585/cmy.72101
  50. Mustafin K.G., Bisko N.A., Achmetsadykov N.K., Sulimenova Zh.B., Zakipbekova A.S., Narmuratova Zh.B. Determination of amino acid and fatty acid composition of depth mycelium Lentinus edodes // Ecology, Environment & Conservation. – 2020. – V. 26, № 2. – P. 931-934. ISSN 0971-765Х.
  51. Mustafin K.G., Biysko N.A., Saduyeva Zh.K., Zhakipbekova A.S., Tapenbayeva I.E. and Narmuratova Zh.B. Chemical composition of depth Lentinus edodes mycelia biomass // Annals of Biology. – 2020. – V. 36, № 3. – P. 463-465.
  52. Poyedinok N.L., Mykchaylova O.B., Sergiichuk N.N., Tugay T.I., Tugay A.V., Lopatko S., Matvieieva N. Effect of Colloidal Metal Nanoparticles on Biomass, Polysaccharides, Flavonoids, and Melanin Accumulation in Medicinal Mushroom Inonotus obliquus (Ach.: Pers.) Pilát. // Applied Biochemistry and Biotechnology. – 2020. – V. 191. – P. 1315-1325.
  53. Prydiuk V.P., Lomberg M.L., First record of Coprinopsis strossmayeri (Psathyrellaceae) in Ukraine: morphological and cultural features // Czech Mycol. – 2021. – V. 73, № 1. – P. 45-58. DOI: 10.33585/cmy.73104
  54. Vedenicheva N., Al-Maali G., Bisko N., Kosakivska I., Ostrovska G., Khanovska N., Gorbach O., Garmanchuk L., Ostapchenko L. Effect of cytokinin-containing extracts from some medicinal mushroom mycelia on HepG2 cells in vitro // Int. J. of Med. Mush. – 2021. – V. 23, № 3. – P. 15-28.